ZNF638 Recombinant antibody

• 

  WB analysis using 84359-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84359-3-RR (ZNF638 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 84359-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF638 antibody (84359-3-RR, Clone: 241636C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84359-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84359-3-RR against Human ZNF638 were performed. The affinity constant is below 1 pM.

ZNF638 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84359-3-RR

Clone No.241636C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

zinc finger protein 638, ZFML, Nuclear protein 220, NP220, CTCL associated antigen se33 1

---

Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, HepG2 cells, HeLa cells, A431 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84359-3-RR targets ZNF638 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZNF638 fusion protein Ag35296
• Full Name: zinc finger protein 638
• Calculated Molecular Weight: 221kDa
• Observed Molecular Weight: 270-300 kDa
• GenBank Accession Number: NM_014497.4
• Gene Symbol: ZNF638
• Gene ID (NCBI): 27332
• RRID: AB_3671896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14966
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404).