ZNF618 Recombinant antibody

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  WB analysis using 84998-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84998-1-RR (ZNF618 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84998-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84998-1-RR against Human ZNF618 were performed. The affinity constant is 1.64 nM.

ZNF618 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84998-1-RR

Clone No.242566D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242566D9, KIAA1952, Zinc finger protein 618

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, MCF-7 cells, Caki-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84998-1-RR targets ZNF618 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZNF618 fusion protein Ag36487
• Full Name: zinc finger protein 618
• Calculated Molecular Weight: 105kDa，954aa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: NM_001318042.1
• Gene Symbol: ZNF618
• Gene ID (NCBI): 114991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T7W0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.