ZNF521 Polyclonal antibody

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  WB analysis using 23716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23716-1-AP (ZNF521 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 23716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF521 antibody (23716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ZNF521 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23716-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Zfp521, zinc finger protein 521, LYST interacting protein 3, LIP3, Evi3

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Tested Applications

• Positive WB detected in: HuH-7 cells, L02 cells, T2 cells, THP-1 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23716-1-AP targets ZNF521 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF521 fusion protein Ag20540
• Full Name: zinc finger protein 521
• Calculated Molecular Weight: 1311 aa, 148 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: BC113648
• Gene Symbol: ZNF521
• Gene ID (NCBI): 25925
• RRID: AB_3669415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96K83
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 521 (EHZF/ZNF521) is a transcription co-factor originally identified for its abundant and selective expression in early progenitors of the human hematopoietic system. In the hematopoietic system ZNF521 is highly expressed in stem and progenitor cells but not in more differentiated precursors or mature leukocytes (PMID: 23907569, 21593590).