ZNF446 Polyclonal antibody

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  WB analysis of SH-SY5Y using 16218-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16218-1-AP (ZNF446 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16218-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16218-1-AP (ZNF446 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 16218-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 16218-1-AP (ZNF446 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ZNF446 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16218-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ZSCAN30, ZKSCAN20

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16218-1-AP targets ZNF446 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF446 fusion protein Ag9266
• Full Name: zinc finger protein 446
• Calculated Molecular Weight: 422 aa, 46 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC017206
• Gene Symbol: ZNF446
• Gene ID (NCBI): 55663
• RRID: AB_2878231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NWS9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Elife; The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.; Authors - Zane A Gibbs