ZNF410 Polyclonal antibody

• 

  WB analysis using 14529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14529-1-AP (ZNF410 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14529-1-AP (ZNF410 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

ZNF410 Polyclonal Antibody for WB, ELISA

Cat No. 14529-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ChIP, ELISA

Synonyms

Zinc finger protein APA 1, Zinc finger protein 410, APA1, APA 1, Another partner for ARF 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse brain tissue, HeLa cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• ChIP: See 1 publications below

Product Information

14529-1-AP targets ZNF410 in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF410 fusion protein Ag6028
• Full Name: zinc finger protein 410
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52-67 kDa
• GenBank Accession Number: BC050683
• Gene Symbol: ZNF410
• Gene ID (NCBI): 57862
• RRID: AB_2257520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VK4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF410 (Zinc Finger Protein 410) is a transcription factor involved in the regulation of gene expression, particularly in the context of fetal hemoglobin (HbF) repression and chromatin remodeling. This protein is highly conserved among mammals and plays a crucial role in erythroid cells. The study found that ZNF410 uniquely silences HbF by activating its sole target gene, CHD4, a known HbF repressor in erythroid cells. (PMID: 40158221)

Publications

Species	Application	Title
human	WB	Nat Genet; ZNF410 represses fetal globin by singular control of CHD4.; Authors - Divya S Vinjamur ; KO Validated
human	WB	Mol Cell; ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.; Authors - Xianjiang Lan
mouse	WB	EMBO J; PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.; Authors - Xixi Tao
human	WB	Mol Ther; Development of a double shmiR lentivirus effectively targeting both BCL11A and ZNF410 for enhanced induction of fetal hemoglobin to treat β-hemoglobinopathies.; Authors - Boya Liu
human	WB	Biomedicines; Heteronemin Suppresses Lymphangiogenesis through ARF-1 and MMP-9/VE-Cadherin/Vimentin.; Authors - Hsien-Lin Chen
mouse	WB	Free Radic Biol Med; Critical role of caveolin-1 in intestinal ischemia reperfusion by inhibiting protein kinase C βII; Authors - Zhao Chen