ZNF395 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZNF395 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11759-1-AP

Synonyms

HD-regulating factor 2, HDBP-2, HDBP2, HDBP 2, HD regulating factor 2

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Validation Data Gallery

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  WB analysis of human brain using 11759-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11759-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11759-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11759-1-AP (ZNF395 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11759-1-AP

  Immunofluorescent analysis of HepG2 cells using 11759-1-AP (ZNF395 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

11759-1-AP targets ZNF395 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF395 fusion protein Ag2355
• Full Name: zinc finger protein 395
• Calculated Molecular Weight: 513 aa, 55 kDa
• Observed Molecular Weight: 62 kDa, 72 kDa
• GenBank Accession Number: BC001237
• Gene Symbol: ZNF395
• Gene ID (NCBI): 55893
• RRID: AB_2218065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].

Publications

Species	Application	Title
human	WB	Onco Targets Ther; Dysregulation of Zinc Finger Protein 395 Contributes to the Pathogenesis of Chondrosarcoma.; Authors - Changbao Chen
human	WB,IHC	Pathobiology; Downregulation of ZNF395 Drives Progression of Pancreatic Ductal Adenocarcinoma through Enhancement of Growth Potential.; Authors - Shusaku Kurogi
human	WB	J Exp Clin Cancer Res; HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395.; Authors - Guoliang Sun ; KD Validated