ZNF346 Polyclonal antibody

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• KD/KO Validated

ZNF346 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20794-1-AP

Synonyms

Zinc finger protein 346, Zfp346, JAZ

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Validation Data Gallery

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  WB analysis of human brain using 20794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20794-1-AP (ZNF346 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20794-1-AP (ZNF346 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 20794-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20794-1-AP (ZNF346 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 20794-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20794-1-AP (ZNF346 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 20794-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20794-1-AP (ZNF346 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 20794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF346 antibody (20794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human testis tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

20794-1-AP targets ZNF346 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF346 fusion protein Ag14585
• Full Name: zinc finger protein 346
• Calculated Molecular Weight: 294 aa, 33 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC007775
• Gene Symbol: ZNF346
• Gene ID (NCBI): 23567
• RRID: AB_10695766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; ZNF385A and ZNF346 Serve as Prognostic Biomarkers Associated with an Inflamed Immunosuppressive Tumor Microenvironment in Hepatocellular Carcinoma; Authors - Qihang Peng ; KD Validated