ZNF326 Polyclonal antibody

ZNF326 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 25147-1-AP

Synonyms

ZAN75, Zfp326, zinc finger protein 326, ZNF326

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Validation Data Gallery

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  WB analysis of Jurkat using 25147-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25147-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25147-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25147-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 25147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse liver using 25147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 25147-1-AP

  Immunofluorescent analysis of HeLa cells using 25147-1-AP (ZNF326 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25147-1-AP is ZNF326 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF326 fusion protein Ag18433
• Full Name: zinc finger protein 326
• Calculated Molecular Weight: 582 aa, 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC090866
• Gene Symbol: ZNF326
• Gene ID (NCBI): 284695
• RRID: AB_2879923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Sci Rep; TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.; Authors - S Prpar Mihevc
mouse	WB	Toxicol Sci; LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5.; Authors - Hongyu Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody detect the band of the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

ZNF326 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:25147-1-AP)