ZNF311 Polyclonal antibody

ZNF311 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23243-1-AP

Synonyms

zf31, ZFP31, zinc finger protein 311, Zinc finger protein zfp 31, ZNF311

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Validation Data Gallery

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  WB analysis of HL-60 using 23243-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 23243-1-AP (ZNF311 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 23243-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 23243-1-AP (ZNF311 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23243-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 23243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 23243-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 23243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23243-1-AP is ZNF311 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF311 fusion protein Ag19624
• Full Name: zinc finger protein 311
• Calculated Molecular Weight: 666 aa, 76 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC136745
• Gene Symbol: ZNF311
• Gene ID (NCBI): 282890
• RRID: AB_11232217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 311 (ZNF311), also named Zinc finger protein zfp-31 (ZFP31), is involved in regulation of transcription. It belongs to the krueppel C2H2-type zinfinger protein family, and contains 14 C2H2-type zinc fingers and 1 KRAB domain.This antibody specifically reacts with ZNF311 protein.