ZNF30 Polyclonal antibody

ZNF30 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24952-1-AP

Synonyms

KOX28, zinc finger protein 30, ZNF30, ZNF30 protein

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Validation Data Gallery

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  WB analysis of mouse spleen using 24952-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24952-1-AP (ZNF30 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24952-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24952-1-AP (ZNF30 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24952-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24952-1-AP (ZNF30 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 24952-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24952-1-AP (ZNF30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24952-1-AP is ZNF30 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF30 fusion protein Ag20090
• Full Name: zinc finger protein 30
• Calculated Molecular Weight: 623 aa, 71 kDa
• Observed Molecular Weight: 65-71 kDa
• GenBank Accession Number: BC142996
• Gene Symbol: ZNF30
• Gene ID (NCBI): 90075
• RRID: AB_2879817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.