ZNF253 Polyclonal antibody

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  WB analysis of HepG2 using 25152-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25152-1-AP (ZNF253 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 25152-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25152-1-AP (ZNF253 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZNF253 Polyclonal Antibody for WB, ELISA

Cat No. 25152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BMZF 1, BMZF1, Bone marrow zinc finger 1, zinc finger protein 253, Zinc finger protein 411, ZNF253, ZNF411

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Tested Applications

• Positive WB detected in: HepG2 cells, SMMC-7721 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25152-1-AP targets ZNF253 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF253 fusion protein Ag18570
• Full Name: zinc finger protein 253
• Calculated Molecular Weight: 499 aa, 58 kDa
• Observed Molecular Weight: 58-65 kDa
• GenBank Accession Number: BC125063
• Gene Symbol: ZNF253
• Gene ID (NCBI): 56242
• RRID: AB_2879927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75346
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF253, also named as Zinc finger protein 411 or BMZF1, is a 499 amino acid protein, which contains one KRAB domain and eleven C2H2-type zinc fingers. ZNF253 belongs to the krueppel C2H2-type zinc-finger protein family and is expressed in bone marrow and in monocytic and immature erythroid cell lines. ZNF253 may function as a transcription factor.