ZNF24 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZNF24 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11219-1-AP

Synonyms

Zinc finger protein 191, Zinc finger and SCAN domain-containing protein 3, Zfp191, RSG-A, RSG A

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Validation Data Gallery

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  WB analysis of HeLa using 11219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11219-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11219-1-AP

  Immunofluorescent analysis of HeLa cells using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11219-1-AP targets ZNF24 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF24 fusion protein Ag1724
• Full Name: zinc finger protein 24
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC016801
• Gene Symbol: ZNF24
• Gene ID (NCBI): 7572
• RRID: AB_10888626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 24 (ZNF24; also known as KOX17, Zfp191 or ZNF191) is a member of the Kruppel-like zinc finger (ZF) transcription factor family, which plays a critical role in cell proliferation and differentiation. There're two isoforms with calculated MW 42KDa and 22KDa. ZNF24 protein can be modificated with SUMO.

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway.; Authors - Wei Li ; KD Validated
human	IHC,WB,IF	Front Oncol; ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation; Authors - Daqi Jia
human	IHC	Exp Cell Res; Tumor-suppressive zinc finger protein 24 (ZNF24) sensitizes colorectal cancer cells to 5-fluorouracil by inhibiting the Wnt pathway and activating the p53 signaling; Authors - Fanqi Meng