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ZNF238/ZBTB18 Polyclonal antibody

ZNF238/ZBTB18 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 12714-1-AP

Synonyms

58 kDa repressor protein, C2H2 171, RP58, TAZ 1, TAZ1, Transcriptional repressor RP58, ZBTB18, ZFP238, zinc finger protein 238, Zinc finger protein C2H2 171, ZNF238



Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, rat heart tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12714-1-AP targets ZNF238/ZBTB18 in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF238/ZBTB18 fusion protein Ag3406
Full Name zinc finger protein 238
Calculated Molecular Weight 531 aa, 59 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC036677
Gene Symbol ZNF238/ZBTB18
Gene ID (NCBI) 10472
RRIDAB_2218388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).

Protocols

Product Specific Protocols
WB protocol for ZNF238/ZBTB18 antibody 12714-1-APDownload protocol
IHC protocol for ZNF238/ZBTB18 antibody 12714-1-APDownload protocol
IP protocol for ZNF238/ZBTB18 antibody 12714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mousechIP

Neuron

FOXG1 Orchestrates Neocortical Organization and Cortico-Cortical Connections.

Authors - Francesca Cargnin
mouseIF

PLoS Biol

Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics.

Authors - Andreas Sagner
mouseIHC

Cereb Cortex

The Zinc Finger Transcription Factor RP58 Negatively Regulates Rnd2 for the Control of Neuronal Migration During Cerebral Cortical Development.

Authors - Heng Julian Ik-Tsen JI
humanWB,CoIP

iScience

Calpain-mediated cleavage generates a ZBTB18 N-terminal product that regulates HIF1A signaling and glioblastoma metabolism.

Authors - Anie P Masilamani
MOUSEIF

Neural Dev

Rp58 and p27(kip1) coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex.

Authors - Olivier Clément
humanWB,RIP

MedComm (2020)

LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58

Authors - Yuanbing Chen