ZNF238/ZBTB18 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZNF238/ZBTB18 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12714-1-AP

Synonyms

58 kDa repressor protein, C2H2 171, RP58, TAZ 1, TAZ1, Transcriptional repressor RP58, ZBTB18, ZFP238, zinc finger protein 238, Zinc finger protein C2H2 171, ZNF238

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Validation Data Gallery

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  WB analysis using 12714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12714-1-AP

  IP result of anti-ZNF238/ZBTB18 (IP:12714-1-AP, 4ug; Detection:12714-1-AP 1:3000) with rat brain tissue lysate 1880 ug.

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  IHC staining of mouse brain using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12714-1-AP (ZNF238 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12714-1-AP (ZNF238 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse skin using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12714-1-AP (ZNF238 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12714-1-AP (ZNF238 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, rat heart tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Product Information

12714-1-AP targets ZNF238/ZBTB18 in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF238/ZBTB18 fusion protein Ag3406
• Full Name: zinc finger protein 238
• Calculated Molecular Weight: 531 aa, 59 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC036677
• Gene Symbol: ZNF238/ZBTB18
• Gene ID (NCBI): 10472
• RRID: AB_2218388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).

Publications

Species	Application	Title
mouse	chIP	Neuron; FOXG1 Orchestrates Neocortical Organization and Cortico-Cortical Connections.; Authors - Francesca Cargnin
mouse	IF	PLoS Biol; Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics.; Authors - Andreas Sagner
mouse	IHC	Cereb Cortex; The Zinc Finger Transcription Factor RP58 Negatively Regulates Rnd2 for the Control of Neuronal Migration During Cerebral Cortical Development.; Authors - Heng Julian Ik-Tsen JI
human	WB,CoIP	iScience; Calpain-mediated cleavage generates a ZBTB18 N-terminal product that regulates HIF1A signaling and glioblastoma metabolism.; Authors - Anie P Masilamani
MOUSE	IF	Neural Dev; Rp58 and p27(kip1) coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex.; Authors - Olivier Clément
human	WB,RIP	MedComm (2020); LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58; Authors - Yuanbing Chen