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ZNF211 Polyclonal antibody

ZNF211 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25458-1-AP

Synonyms

C2H2 25, CH2H2 25, zinc finger protein 211, Zinc finger protein C2H2 25, ZNF 25, ZNF211, ZNFC25

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF211 Polyclonal antibody (25458-1-AP)

    WB analysis of HeLa using 25458-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25458-1-AP (ZNF211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF211 Polyclonal antibody (25458-1-AP)

    WB analysis of Jurkat using 25458-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25458-1-AP (ZNF211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25458-1-AP is ZNF211 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF211 fusion protein Ag22135
Full Name zinc finger protein 211
Calculated Molecular Weight 576 aa, 66 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC110856
Gene Symbol ZNF211
Gene ID (NCBI) 10520
RRIDAB_2880090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF211 antibody 25458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Daniel (Verified Customer) (10-26-2022)

This antibody works at low dilutions and using PVDF only.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T, MT4 cells
ZNF211 Antibody Western Blot validation (1:500 dilution) in HEK293T, MT4 cells (Cat no:25458-1-AP)