ZNF148 Recombinant antibody

• 

  WB analysis using 84338-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84338-7-RR (ZNF148 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84338-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84338-7-RR against Human ZNF148 were performed. The affinity constant is 1.85 nM.

ZNF148 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84338-7-RR

Clone No.241632D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Transcription factor ZBP 89, pHZ 52, HT BETA, BFCOL1, BERF 1

---

Tested Applications

• Positive WB detected in: MCF-7 cells, T-47D cells, HCT 116 cells, HeLa cells, MDA-MB-231 cells, HepG2 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84338-7-RR targets ZNF148 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZNF148 fusion protein Ag35742
• Full Name: zinc finger protein 148
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC035591
• Gene Symbol: ZNF148
• Gene ID (NCBI): 7707
• RRID: AB_3671879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UQR1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. ZNF148 participates in the regulation of cell proliferation and death, and is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells. Previous studies show that there was a significant correlation between ZNF148 expression and the invasion and metastasis of colorectal tumors. ZNF148 has two alternative splicing isoforms, which are ZNF148FL and ZNF148ΔN. ZNF148FL contains a complete 794 amino acids, ZNF148ΔN lacks the amino-terminal 129 amino acids, and the mechanisms involved in the generation of these two splicing isoforms are not yet clear (PMID: 30463804). Thus, ZNF148 can be observed to have two molecular weights near 103 kDa.