ZMYND8 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZMYND8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11633-1-AP

Synonyms

CTCL associated antigen se14 3, KIAA1125, PRKCBP1, PRO2893, RACK7, ZMYND8

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 11633-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 11633-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11633-1-AP

  IP result of anti-ZMYND8 (IP:11633-1-AP, 3ug; Detection:11633-1-AP 1:300) with HeLa cells lysate 920ug.

• 

  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

11633-1-AP targets ZMYND8 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZMYND8 fusion protein Ag2207
• Full Name: zinc finger, MYND-type containing 8
• Calculated Molecular Weight: 1186 aa, 132 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC030721
• Gene Symbol: ZMYND8
• Gene ID (NCBI): 23613
• RRID: AB_2241834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins including REST corepressors (RCOR). ZMYND8 bind to ROCR2 and can function as transcriptional repressors. It is co-expressed with RCOR2 in the nervous system and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]

Publications

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
mouse,human		Mol Cell; The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.; Authors - Qiang Pan ; KO Validated
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
human	WB	Cancer Res; KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity; Authors - Yulong Qiang
human	WB	Mol Cell; Basis of gene-specific transcription regulation by the Integrator complex; Authors - Kevin Sabath
human	WB,IHC,IF	Sci Adv; The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation.; Authors - Xi Jin