ZIC4-Specific Polyclonal antibody

ZIC4-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 51003-1-AP

Synonyms

Zic family member 4, ZIC4, Zinc finger protein ZIC 4

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Validation Data Gallery

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  WB analysis of human brain using 51003-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 51003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 51003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

51003-1-AP targets ZIC4-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Zic family member 4
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_032153
• Gene Symbol: ZIC4
• Gene ID (NCBI): 84107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ZIC gene family includes 5 genes that are highly conserved across evolution. These genes encode zinc finger proteins that are expressed during development and maturation of the central nervous system and have critical roles in the development of the cerebellum. ZIC4, likes other member, may has roles in the nervous system development.

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Neural Tube Defects and ZIC4 Hypomethylation in Relation to Polycyclic Aromatic Hydrocarbon Exposure.; Authors - Yun Huang
human	WB,IHC	BMC Cancer; Expression of ZIC family genes in meningiomas and other brain tumors.; Authors - Aruga Jun J