ZIC1 Polyclonal antibody

ZIC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25004-1-AP

Synonyms

ZIC, ZIC1, Zinc finger protein ZIC 1, ZNF201

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Validation Data Gallery

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  WB analysis using 25004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25004-1-AP (ZIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 25004-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25004-1-AP (ZIC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: ROS1728 cells, Y79 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25004-1-AP targets ZIC1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZIC1 fusion protein Ag13400
• Full Name: Zic family member 1 (odd-paired homolog, Drosophila)
• Calculated Molecular Weight: 447 aa, 48 kDa
• Observed Molecular Weight: ~50 kDa
• GenBank Accession Number: BC104848
• Gene Symbol: ZIC1
• Gene ID (NCBI): 7545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZIC1 is a member of the Zinc finger of the cerebellum (ZIC) protein family. In vertebrates, the ZIC gene family plays an important role in neural crest and subsequent cerebellar development. Mutations in ZIC family members have demonstrated that they are essential for proper development of the neural tube, left-right symmetry, and muscle and skeletal development. It has been reported that ZIC1 participates in the regulation of cell proliferation in the dorsal neural tube by inhibition of neural differentiation. Furthermore, ZIC1 is potentially involved in the oncogenesis of undifferentiated nervous system tumors. (PMID:17936758)