ZHX2 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZHX2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4E12F2

Cat no : 68268-1-Ig

Synonyms

RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 68268-1-Ig

  WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells.

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  WB analysis using 68268-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of A431 using 68268-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZHX2 antibody (68268-1-Ig, Clone: 4E12F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68268-1-Ig targets ZHX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZHX2 fusion protein Ag14420
• Full Name: zinc fingers and homeoboxes 2
• Calculated Molecular Weight: 837 aa, 92 kDa
• Observed Molecular Weight: 92-100 kDa
• GenBank Accession Number: BC042145
• Gene Symbol: ZHX2
• Gene ID (NCBI): 22882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.