ZG16 Monoclonal antibody, PBS Only

ZG16 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7B9

Cat no : 67389-1-PBS

Synonyms

FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

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Validation Data Gallery

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  WB analysis of mouse colon using 67389-1-Ig (same clone as 67389-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 67389-1-Ig (same clone as 67389-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 67389-1-Ig (same clone as 67389-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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Product Information

67389-1-PBS targets ZG16 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZG16 fusion protein Ag11332
• Full Name: zymogen granule protein 16 homolog (rat)
• Calculated Molecular Weight: 167 aa, 18 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC029149
• Gene Symbol: ZG16
• Gene ID (NCBI): 653808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)