Rabenosyn 5 Polyclonal antibody

Rabenosyn 5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22218-1-AP

Synonyms

110 kDa protein, Rabenosyn 5, ZFYVE20

---

Validation Data Gallery

• 

  WB analysis of K-562 using 22218-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22218-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22218-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22218-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22218-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22218-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 22218-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: K-562 cells, A549 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

The immunogen of 22218-1-AP is Rabenosyn 5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Rabenosyn 5 fusion protein Ag17554
• Full Name: zinc finger, FYVE domain containing 20
• Calculated Molecular Weight: 784 aa, 89 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC106940
• Gene Symbol: Rabenosyn 5
• Gene ID (NCBI): 64145
• RRID: AB_11182179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
human	WB, IHC	J Mol Biol; Identification of New Interactions between Endolysosomal Tethering Factors.; Authors - Zsófia Simon-Vecsei
mouse	WB,IF,IP	Int J Mol Sci; Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage; Authors - Masato Mashimo
human	WB,IF	Nat Commun; Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic; Authors - Seiichi Koike