ZFP36L2 Recombinant antibody

• 

  WB analysis using 85154-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85154-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85154-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

ZFP36L2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85154-4-RR

Clone No.242776C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, ELISA

Synonyms

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

---

Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, Jurkat cells, MCF-7 cells, HepG2 cells, COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85154-4-RR targets ZFP36L2 in WB, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZFP36L2 fusion protein Ag13515
• Full Name: zinc finger protein 36, C3H type-like 2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC005010
• Gene Symbol: ZFP36L2
• Gene ID (NCBI): 678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47974
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).