ZFP36L1 Monoclonal antibody

ZFP36L1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6G6

Cat no : 68038-1-Ig

Synonyms

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

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Validation Data Gallery

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  WB analysis using 68038-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HSC-T6 using 68038-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 68038-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68038-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFP36L1 antibody (68038-1-Ig, Clone: 1H6G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, HSC-T6 cells, PC-12 cells, U2OS cells, A549 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68038-1-Ig targets ZFP36L1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZFP36L1 fusion protein Ag30469
• Full Name: zinc finger protein 36, C3H type-like 1
• Calculated Molecular Weight: 338 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC018340
• Gene Symbol: ZFP36L1
• Gene ID (NCBI): 677
• RRID: AB_2918780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.