ZFP36L1/2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZFP36L1/2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12306-1-AP

Synonyms

ZFP36L1, ZFP36 L1, TPA-induced sequence 11b, mRNA decay activator protein ZFP36L1, ERF-1

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Validation Data Gallery

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  WB analysis of U-937 using 12306-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12306-1-AP

  WB result of ZFP36L1/2 antibody (12306-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZFP36L1/2 transfected HeLa cells.

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  WB analysis of RAW 264.7 using 12306-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 12306-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 12306-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat ovary using 12306-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 12306-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L1/2 antibody (12306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: U-937 cells, 3T3-L1 cells, RAW 264.7 cells, HeLa cells
• Positive IHC detected in: rat ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

12306-1-AP targets ZFP36L1/2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFP36L1/2 fusion protein Ag2952
• Full Name: zinc finger protein 36, C3H type-like 1
• Calculated Molecular Weight: 338 aa, 36 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC018340
• Gene Symbol: ZFP36L1
• Gene ID (NCBI): 677
• RRID: AB_2737443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFP36L1, also named as Butyrate response factor 1 or TPA-induced sequence 11b, is a 338 amino acid protein, which is phosphorylated by RPS6KA1 at Ser-334 upon phorbol 12-myristate 13-acetate (PMA) treatment. ZFP36 encodes tristetraprolin (TTP) the prototype of a small family of RBPs, called the ZFP36 family, that are characterised by highly conserved tandem CCCH zinc-finger RNA-binding domains [PMID: 10751406]. ZFP36 is a RBP that promotes RNA decay and negatively regulates the expression of the myogenic regulatory factor MyoD by binding to the 3'UTR of MyoD mRNA [PMID: 25815583]. Mouse satellite cells from Zfp36-deficient mice express increased amounts of MyoD and display impaired satellite activation, demonstrating a role for ZFP36 in the maintenance of quiescence [PMID: 25815583]. The functions of the ZFP36L1 and ZFP36L2 family members have not been evaluated in skeletal muscle stem cell fate, but have been shown to act redundantly to promote quiescence during lymphocyte development. ZFP36L1 has been implicated in the persistence of the marginal zone B lymphocyte population. ZFP36L1 exists three isoform through blasting on NCBI database and can be phosphorylated, so the range of the molecular weight of ZFP36L1 is about 40-50 kDa. (PMID: 26180518, PMID: 17030620). This antibody recognizes both ZFP36L1 and ZFP36L2.

Publications

Species	Application	Title
human	IF	Cell; A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3'UTR-Mediated Protein-Protein Interactions.; Authors - Weirui Ma
human	IF	Mol Ther Nucleic Acids; The miR-93-3p/ZFP36L1/ZFX axis regulates keratinocyte proliferation and migration during skin wound healing.; Authors - Xiao Feng ; KD Validated
mouse	WB,IHC,IF	Cell Death Dis; ZFP36L2 regulates myocardial ischemia/reperfusion injury and attenuates mitochondrial fusion and fission by LncRNA PVT1.; Authors - Fang Wu
human	WB	J Clin Invest; EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer; Authors - Xiaochao Tan ; KD Validated
human	WB	Elife; HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation; Authors - Sara Scinicariello
human	WB,IHC,IF	Heliyon; BRF1 promotes the odontogenic differentiation of dental pulp stem cells in pulpitis by inducing autophagy; Authors - Caixia Zhou