ZFP36 Polyclonal antibody

ZFP36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 12737-1-AP

Synonyms

TTP, Zfp-36, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36

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Validation Data Gallery

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  WB analysis using 12737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12737-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12737-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human bladder using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 12737-1-AP

  Immunofluorescent analysis of HepG2 cells using 12737-1-AP (ZFP36 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse lung tissue, A549 cells, rat lung tissue
• Positive IHC detected in: human breast cancer tissue, human bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 4 publications below

Product Information

12737-1-AP targets ZFP36 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFP36 fusion protein Ag3461
• Full Name: zinc finger protein 36, C3H type, homolog (mouse)
• Calculated Molecular Weight: 326 aa, 34 kDa
• Observed Molecular Weight: 37-44 kDa
• GenBank Accession Number: BC009693
• Gene Symbol: ZFP36
• Gene ID (NCBI): 7538
• RRID: AB_10598485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF). The calcualted molecular weight of ZFP36 is 34 kDa, but modified ZFP36 is about 40-45 kDa.

Publications

Species	Application	Title
		Mol Cell; Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.; Authors - Ameya P Jalihal
mouse	WB,RIP	Dev Cell; N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.; Authors - Jie Du
		Proc Natl Acad Sci U S A; Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.; Authors - Frederick E Tan
human	WB,RIP	Am J Physiol Cell Physiol; Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1; Authors - Jie Zhao
human	WB	Sci Rep; The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.; Authors - Yuan Wang
human	WB	Oncotarget; Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.; Authors - Bo Li