ZFP161 / ZBTB14 Recombinant antibody

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  WB analysis using 83909-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83909-4-RR (ZFP161 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 83909-4-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR, Clone:240853B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83909-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83909-4-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is below 1 pM.

ZFP161 / ZBTB14 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83909-4-RR

Clone No.240853B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

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Tested Applications

• Positive WB detected in: HEK-293T cells, L02 cells, Daudi cells, Jurkat cells, K-562 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83909-4-RR targets ZFP161 / ZBTB14 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZFP161 / ZBTB14 fusion protein Ag16125
• Full Name: zinc finger protein 161 homolog (mouse)
• Calculated Molecular Weight: 449 aa, 51 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC110519
• Gene Symbol: ZFP161
• Gene ID (NCBI): 7541
• RRID: AB_3671490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43829
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.