ZEB1 Monoclonal antibody, PBS Only

ZEB1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H1F1

Cat no : 66279-1-PBS

Synonyms

AREB6, BZP, DELTA EF1, delta EF1,, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A

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Validation Data Gallery

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  WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66279-1-Ig (same clone as 66279-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66279-1-Ig (same clone as 66279-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SKOV-3 using 66279-1-Ig (same clone as 66279-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66279-1-Ig (same clone as 66279-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66279-1-Ig (same clone as 66279-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66279-1-Ig (same clone as 66279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66279-1-Ig (same clone as 66279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66279-1-PBS targets ZEB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZEB1 fusion protein Ag17118
• Full Name: zinc finger E-box binding homeobox 1
• Calculated Molecular Weight: 1125 aa, 124 kDa
• Observed Molecular Weight: 190-200 kDa
• GenBank Accession Number: BC112392
• Gene Symbol: ZEB1
• Gene ID (NCBI): 6935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa.