ZEB1 Polyclonal antibody

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  WB analysis using 21544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21544-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 21544-1-AP

  IP result of anti-ZEB1 (IP:21544-1-AP, 4ug; Detection:21544-1-AP 1:8000) with COLO 320 cells lysate 1320 ug.

• Featured Product
• KD/KO Validated

ZEB1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 21544-1-AP

Publications(246)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, chIP, ELISA

Synonyms

AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, PC-3 cells, COLO 320 cells, MDA-MB-453s cells, mouse colon tissue
• Positive IP detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 22 publications below
• WB: See 223 publications below
• IHC: See 29 publications below
• IF: See 22 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below
• ChIP: See 13 publications below

Product Information

21544-1-AP targets ZEB1 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZEB1 fusion protein Ag16204
• Full Name: zinc finger E-box binding homeobox 1
• Calculated Molecular Weight: 1125 aa, 124 kDa
• Observed Molecular Weight: 190-210 kDa
• GenBank Accession Number: BC112392
• Gene Symbol: ZEB1
• Gene ID (NCBI): 6935
• RRID: AB_10734325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P37275
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Publications

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA; Authors - Ya-Rui Hou
human	chIP	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
human	WB	Nat Commun; Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63.; Authors - Yingjie Liu
human	WB,IP,ChIP	Nat Commun; Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasis.; Authors - Nastaran Mohammadi Ghahhari
human	WB, ChIP	J Exp Med; Squamous cell carcinoma subverts adjacent histologically normal epithelium to promote lateral invasion.; Authors - Priyanka Singh
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (10-14-2021)

Antibody worked really well first time and produced clear Zeb1 bands at the MW expected, with barely any non specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVECs

FH

K (Verified Customer) (12-20-2020)

I used tis Ab for western blotting at 1:2000 dilution and got good results.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: human liver cells