ZDHHC8 Polyclonal antibody

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  WB analysis using 27179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27179-1-AP (ZDHHC8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 27179-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27179-1-AP (ZDHHC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZDHHC8 Polyclonal Antibody for WB, ELISA

Cat No. 27179-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DHHC 8, KIAA1292, ZDHHC8, ZDHHCL1, Zinc finger protein 378, ZNF378

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Tested Applications

• Positive WB detected in: mouse brain tissue, K-562 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

27179-1-AP targets ZDHHC8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZDHHC8 fusion protein Ag26028
• Full Name: zinc finger, DHHC-type containing 8
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC053544
• Gene Symbol: ZDHHC8
• Gene ID (NCBI): 29801
• RRID: AB_2880789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULC8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; AMPKα1-mediated ZDHHC8 phosphorylation promotes the palmitoylation of SLC7A11 to facilitate ferroptosis resistance in glioblastoma; Authors - Zhangjie Wang
human	WB	Sci Rep; Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages; Authors - Ryosuke Shimai