ZDHHC5 Recombinant antibody

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  WB analysis using 84803-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 84803-4-RR

  IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug.

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  Affinity and Kinetic Characterization of 84803-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

ZDHHC5 {ptg:Brand} Recombinant Antibody for WB, IP, ELISA

Cat No. 84803-4-RR

Clone No.242192A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Zinc finger DHHC domain-containing protein 5, Palmitoyltransferase ZDHHC5, KIAA1748, EC:2.3.1.225, DHHC-5

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84803-4-RR targets ZDHHC5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZDHHC5 fusion protein Ag15022
• Full Name: zinc finger, DHHC-type containing 5
• Calculated Molecular Weight: 715 aa, 78 kDa
• Observed Molecular Weight: 75-77 kDa
• GenBank Accession Number: BC026967
• Gene Symbol: ZDHHC5
• Gene ID (NCBI): 25921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9C0B5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.