ZDHHC15 Polyclonal antibody

ZDHHC15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21627-1-AP

Synonyms

DHHC 15, MRX91, Palmitoyltransferase ZDHHC15, ZDHHC15

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Validation Data Gallery

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  WB analysis using 21627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 21627-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21627-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 21627-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 21627-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, SMMC-7721 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below

Product Information

21627-1-AP targets ZDHHC15 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZDHHC15 fusion protein Ag16152
• Full Name: zinc finger, DHHC-type containing 15
• Calculated Molecular Weight: 337 aa, 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC103982
• Gene Symbol: ZDHHC15
• Gene ID (NCBI): 158866
• RRID: AB_2878892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	IP	Mol Cell Endocrinol; ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells; Authors - Shanmeizi Zhao