ZDHHC13 Polyclonal antibody

ZDHHC13 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24759-1-AP

Synonyms

DHHC 13, HIP14 related protein, HIP14L, HIP3RP, Palmitoyltransferase ZDHHC13, ZDHHC13

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Validation Data Gallery

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  WB analysis of mouse kidney using 24759-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24759-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 24759-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 24759-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 24759-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24759-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 24759-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24759-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HEK-293 using 24759-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, mouse skin tissue, mouse testis tissue, Neuro-2a cells
• Positive IHC detected in: human stomach tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 24759-1-AP is ZDHHC13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZDHHC13 fusion protein Ag21607
• Full Name: zinc finger, DHHC-type containing 13
• Calculated Molecular Weight: 622 aa, 71 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC056152
• Gene Symbol: ZDHHC13
• Gene ID (NCBI): 54503
• RRID: AB_2879708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms. The molecular weight of isoform 2 is 51 kDa, but we often detected a 45-50kDa protein by western blot.

Publications

Species	Application	Title
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga