ZDHHC12 Polyclonal antibody

ZDHHC12 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26433-1-AP

Synonyms

DHHC 12, ZDHHC12, Zinc finger protein 400, ZNF400

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Validation Data Gallery

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  IHC staining of human kidney using 26433-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26433-1-AP (ZDHHC12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26433-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26433-1-AP (ZDHHC12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 26433-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26433-1-AP (ZDHHC12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26433-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26433-1-AP (ZDHHC12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below

Product Information

26433-1-AP targets ZDHHC12 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZDHHC12 fusion protein Ag23634
• Full Name: zinc finger, DHHC-type containing 12
• Observed Molecular Weight: 29 kDa, 32 kDa
• GenBank Accession Number: BC048251
• Gene Symbol: ZDHHC12
• Gene ID (NCBI): 84885
• RRID: AB_2880512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	Autophagy; ABHD8 antagonizes inflammation by facilitating chaperone-mediated autophagy-mediated degradation of NLRP3; Authors - Shuai Yang