ZCCHC11 Polyclonal antibody

ZCCHC11 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20119-1-AP

Synonyms

KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11

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Validation Data Gallery

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  WB analysis of HeLa using 20119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20119-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20119-1-AP

  Immunofluorescent analysis of Hela cells, using ZCCHC11 antibody 20119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20119-1-AP is ZCCHC11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZCCHC11 fusion protein Ag13661
• Full Name: zinc finger, CCHC domain containing 11
• Calculated Molecular Weight: 185 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC048301
• Gene Symbol: ZCCHC11
• Gene ID (NCBI): 23318
• RRID: AB_10664927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.

Publications

Species	Application	Title
human	WB,IF	Neoplasia; Identification of novel targets of miR-622 in hepatocellular carcinoma reveals common regulation of cooperating genes and outlines the oncogenic role of zinc finger CCHC-type containing 11.; Authors - Anne Gaza