ZC3HAV1 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZC3HAV1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G10B9

Cat no : 66413-1-Ig

Synonyms

PRO1677, PARP13, Inactive Poly [ADP-ribose] polymerase 13, ARTD13, ADP-ribosyltransferase diphtheria toxin-like 13

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Validation Data Gallery

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  WB analysis of HEK-293 using 66413-1-Ig

  WB result of ZC3HAV1 antibody (66413-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells.

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  WB analysis of Jurkat using 66413-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66413-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66413-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66413-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 40x lens).

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  IHC staining of human kidney using 66413-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 10x lens).

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  IF Staining of HepG2 using 66413-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZC3HAV1 antibody (66413-1-Ig, Clone: 1G10B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

66413-1-Ig targets ZC3HAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZC3HAV1 fusion protein Ag10364
• Full Name: zinc finger CCCH-type, antiviral 1
• Calculated Molecular Weight: 699aa,78 kDa; 902aa,101 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC025308
• Gene Symbol: ZC3HAV1
• Gene ID (NCBI): 56829
• RRID: AB_2881785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

Publications

Species	Application	Title
human	WB	mBio; The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.; Authors - Ana Cristina Gonzalez-Perez ; KO Validated
human	IF	J Virol; Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1.; Authors - Marlene V Buckmaster
human	WB	EMBO Rep; Interferon inhibits a model RNA virus via a limited set of inducible effector genes; Authors - Matthew B McDougal
human	WB	Nat Commun; TRIM25 predominately associates with anti-viral stress granules; Authors - Zehua Shang