ZC3H13 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZC3H13 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E8C5

Cat no : 68526-1-Ig

Synonyms

KIAA0853, 3E8C5

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Validation Data Gallery

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  WB analysis of HEK-293 using 68526-1-Ig

  WB result of ZC3H13 antibody (68526-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H13 transfected HEK-293 cells.

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  WB analysis using 68526-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68526-1-Ig (ZC3H13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68526-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68526-1-Ig (ZC3H13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 68526-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68526-1-Ig (ZC3H13 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 68526-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68526-1-Ig (ZC3H13 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells, HEK-293 cells, A549 cells, MCF-7 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68526-1-Ig targets ZC3H13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZC3H13 fusion protein Ag29132
• Full Name: zinc finger CCCH-type containing 13
• Calculated Molecular Weight: 197 kDa
• Observed Molecular Weight: 196-240 kDa
• GenBank Accession Number: NM_001076788
• Gene Symbol: ZC3H13
• Gene ID (NCBI): 23091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.