ZC3H12A Monoclonal antibody

ZC3H12A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F6B2

Cat no : 68616-1-Ig

Synonyms

dJ423B22.1, FLJ23231, MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A

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Validation Data Gallery

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  WB analysis using 68616-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68616-1-Ig (ZC3H12A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: HT-1376 cells, BxPC-3 cells, MDA-MB-468 cells, Raji cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68616-1-Ig targets ZC3H12A in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZC3H12A fusion protein Ag13877
• Full Name: zinc finger CCCH-type containing 12A
• Calculated Molecular Weight: 599 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC005001
• Gene Symbol: ZC3H12A
• Gene ID (NCBI): 80149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.