ZC3H11A Recombinant antibody

ZC3H11A Uni-rAb^TM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230191B4

Cat no : 82912-3-RR

Synonyms

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191B4

---

Validation Data Gallery

• 

  FC experiment of MCF-7 using 82912-3-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human ZC3H11A (82912-3-RR, Clone:230191B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of A431 using 82912-3-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human ZC3H11A (82912-3-RR, Clone:230191B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 82912-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82912-3-RR against Human ZC3H11A were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive FC (Intra) detected in: A431 cells, MCF-7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82912-3-RR targets ZC3H11A in FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZC3H11A fusion protein Ag23424
• Full Name: zinc finger CCCH-type containing 11A
• GenBank Accession Number: BC014268
• Gene Symbol: ZC3H11A
• Gene ID (NCBI): 9877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.