ZBTB7B Polyclonal antibody

ZBTB7B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28127-1-AP

Synonyms

c Krox, DKFZp686G01254, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B

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Validation Data Gallery

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  WB analysis using 28127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28127-1-AP (ZBTB7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28127-1-AP targets ZBTB7B in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB7B fusion protein Ag27970
• Full Name: zinc finger and BTB domain containing 7B
• Calculated Molecular Weight: 539 aa, 58 kDa
• Observed Molecular Weight: 70 kda
• GenBank Accession Number: BC012070
• Gene Symbol: ZBTB7B
• Gene ID (NCBI): 51043
• RRID: AB_2881068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB7B, also known as Zfp-67 or Zinc finger protein Th-POK, is a 539 amino acid protein, which localizes in nucleus. ZBTB7B as a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. ZBTB7B is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. The calcualted molecular weight of ZBTB7B is about 58 kDa, but modified protein is 70 kDa.