ZBTB7B Polyclonal antibody

ZBTB7B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11341-1-AP

Synonyms

ZFP67, Zfp 67, ZBTB15, THPOK, hcKrox

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Validation Data Gallery

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  WB analysis using 11341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11341-1-AP (ZBTB7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11341-1-AP

  IP result of anti-ZBTB7B (IP:11341-1-AP, 4ug; Detection:11341-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IF Staining of HeLa using 11341-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBTB7B antibody (11341-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

11341-1-AP targets ZBTB7B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB7B fusion protein Ag1888
• Full Name: zinc finger and BTB domain containing 7B
• Calculated Molecular Weight: 539 aa, 58 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC012070
• Gene Symbol: ZBTB7B
• Gene ID (NCBI): 51043
• RRID: AB_2217245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB7B belongs to a large family of transcription factors, generally acting as repressors, characterized by a carboxy-terminal DNA binding domain made of multiple zinc fingers (four in Thpok) and an amino-terminal BTB-POZ domain that mediates homo- (and possibly hetero-) dimerization [PMID: 17084908]. Zbtb7b is up-regulated by MHC-II-restricted thymocytes during their CD4 differentiation and is a major determinant of CD4 lineage choice. Two properties of ZBTB7B deserve attention. First, although Thpok is expressed in a wide variety of cells, its expression in the thymus is highly lineage-specific : CD4 SP thymocytes (and all CD4 T cells) express Thpok, whereas DP and CD8 SP thymocytes do notSecond, both loss- and gain-of-function experiments indicate that Thpok affects lineage choice but not positive selection [PMID: 15729333, 10550051].

Publications

Species	Application	Title
hamster	WB	Acta Biomater; LAP2β transmits force to upregulate genes via chromatin domain stretching but not compression; Authors - Jian Sun
mouse	WB,IHC	J Nanobiotechnology; Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4+CD8+T cells expansion; Authors - Min-Zheng Zhu
human	WB,IHC	J Transl Med; Epigenetic DNA methylation of Zbtb7b regulates the population of double-positive CD4+CD8+ T cells in ulcerative colitis.; Authors - Hao-Ming Xu
bovine	WB,IP	J Agric Food Chem; Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells; Authors - Jiaxin Wang