ZBTB7A Polyclonal antibody

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  WB analysis using 18700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18700-1-AP (ZBTB7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 18700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB7A antibody (18700-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

ZBTB7A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 18700-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

HIV-1 1st-binding protein 1, HIV 1 1st binding protein 1, FBI-1, FBI1, FBI 1

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, NCI-H1299 cells, U2OS cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

18700-1-AP targets ZBTB7A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: zinc finger and BTB domain containing 7A
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61-70 kDa
• GenBank Accession Number: BC113511
• Gene Symbol: ZBTB7A
• Gene ID (NCBI): 51341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95365
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa and the 75~80-kDa band could be the sumoylated form.

Publications

Species	Application	Title
human,mouse	WB	Biomed Res Int; Obesity-Induced Upregulation of ZBTB7A Promotes Lipid Accumulation through SREBP1.; Authors - Jing-Ping Zhou ; KD Validated