ZBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 13285-1-AP

Synonyms

C20orf183, DAI, DLM 1, DLM1, Z DNA binding protein 1, ZBP1

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Validation Data Gallery

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  WB analysis using 13285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13285-1-AP (ZBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

13285-1-AP targets ZBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBP1 fusion protein Ag4119
• Full Name: Z-DNA binding protein 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 42-68 kDa
• GenBank Accession Number: BC028218
• Gene Symbol: ZBP1
• Gene ID (NCBI): 81030
• RRID: AB_2122651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

Publications

Species	Application	Title
human	WB	Sci Rep; Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells.; Authors - Bartosz Szczesny ; KD Validated
human,mouse	WB	Physiol Genomics; PANoptosis in vascular smooth muscle cells regulated by TNF-α/IL-1β can be a new target for alleviating the progression of abdominal aortic aneurysm; Authors - Kun Li
human		J Biol Chem; Oxidative stress induces Z-DNA-binding protein 1-dependent activation of microglia via mtDNA released from retinal pigment epithelial cells.; Authors - Jamal Saada
human	WB	Toxicol Appl Pharmacol; The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells; Authors - Jun Li
human,mouse	WB	mBio; Sperm-Associated Antigen 9 Promotes Influenza A Virus-Induced Cell Death via the c-Jun N-Terminal Kinase Signaling Pathway.; Authors - Rui Gui
mouse	WB	Heliyon; Analysis of the role of PANoptosis in seizures via integrated bioinformatics analysis and experimental validation; Authors - Yueying Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charity (Verified Customer) (02-15-2024)

this will probably work at a lower concentration but I have not tried it yet.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse lung tissue sections

ZBP1 Antibody Immunofluorescence validation (1:100 dilution) in mouse lung tissue sections (Cat no:13285-1-AP)