ZAP70 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZAP70 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B3C6

Cat no : 60200-2-Ig

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

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Validation Data Gallery

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  WB analysis of Jurkat using 60200-2-Ig

  WB result of ZAP70 antibody (60200-2-Ig, 1:5000) with si-control and si-ZAP70 transfected Jurkat cells..

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  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 60200-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 60200-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60200-2-Ig is ZAP70 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZAP70 fusion protein Ag7943
• Full Name: zeta-chain (TCR) associated protein kinase 70kDa
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039039
• Gene Symbol: ZAP70
• Gene ID (NCBI): 7535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.