ZAP70 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZAP70 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 15592-1-AP

Synonyms

Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein

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Validation Data Gallery

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  WB analysis of Jurkat using 15592-1-AP

  WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.

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  WB analysis using 15592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15592-1-AP

  IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of RAW 264.7 using 15592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, A375 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15592-1-AP targets ZAP70 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZAP70 fusion protein Ag7943
• Full Name: zeta-chain (TCR) associated protein kinase 70kDa
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039039
• Gene Symbol: ZAP70
• Gene ID (NCBI): 7535
• RRID: AB_2218654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Publications

Species	Application	Title
human	WB	J Immunol Methods; An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination; Authors - Jiaqi Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

hala (Verified Customer) (01-27-2023)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: hepG2

ZAP70 Antibody Western Blot validation (1/10000 dilution) in hepG2 (Cat no:15592-1-AP)