ZAK Polyclonal antibody

• Featured Product
• KD/KO Validated

ZAK Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 14945-1-AP

Synonyms

AZK, HCCS 4, MAP3K20, Mitogen-activated protein kinase kinase kinase 20, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

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Validation Data Gallery

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  WB analysis using 14945-1-AP

  mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14945-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 14945-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14945-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14945-1-AP (ZAK Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human placenta using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14945-1-AP (ZAK Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 14945-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZAK antibody (14945-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human heart tissue, HepG2 cells, human skeletal muscle tissue
• Positive IHC detected in: human liver cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below

Product Information

14945-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZAK fusion protein Ag6747
• Full Name: sterile alpha motif and leucine zipper containing kinase AZK
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001401
• Gene Symbol: ZAK
• Gene ID (NCBI): 51776
• RRID: AB_10642698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

Publications

Species	Application	Title
human	WB	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang ; KO Validated
human	WB	Sci Signal; Poly-GR repeats associated with ALS/FTD gene C9ORF72 impair translation elongation and induce a ribotoxic stress response in neurons; Authors - Daoyuan Dong
human	WB	bioRxiv; Initiation of a ZAKα-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L; Authors - Jiajia Xi
mouse	WB	Nat Commun; Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation; Authors - Joana Silva
mouse	WB	Cell Metab; Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response; Authors - Goda Snieckute
human	WB	Cell Death Dis; Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms; Authors - Laura Ryder

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amy (Verified Customer) (08-14-2019)

Worked well with 5% milk in 0.2% PBST.Shows up well the beta isoform in mouse muscle, and alpha in heart.Did not work in Zebrafish despite the kinase domain being highly conserved between organisms.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: C2C12 and mouse skeletal muscle tissues