YWHAZ Monoclonal antibody, PBS Only

YWHAZ Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat no : 67698-1-PBS

Synonyms

14 3 3 protein zeta/delta, KCIP 1, YWHAZ

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Validation Data Gallery

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  WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67698-1-Ig (YWHAZ antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67698-1-Ig (same clone as 67698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using YWHAZ antibody (67698-1-Ig, Clone: 3G12B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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Product Information

67698-1-PBS targets YWHAZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: YWHAZ fusion protein Ag6667
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC003623
• Gene Symbol: YWHAZ
• Gene ID (NCBI): 7534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.