YWHAH Polyclonal antibody

• Featured Product
• KD/KO Validated

YWHAH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 15222-1-AP

Synonyms

14-3-3 eta, 14-3-3 protein eta, 14-3-3, 14 3 3 protein eta

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Validation Data Gallery

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  WB analysis of human brain using 15222-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15222-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15222-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15222-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15222-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15222-1-AP

  IP result of anti-YWHAH (IP:15222-1-AP, 4ug; Detection:15222-1-AP 1:1000) with NIH/3T3 cells lysate 1600ug.

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  IHC staining of human skin cancer using 15222-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15222-1-AP (YWHAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15222-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15222-1-AP (YWHAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15222-1-AP

  Immunofluorescent analysis of Hela cells, using YWHAH antibody 15222-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue, A431 cells, mouse brain tissue, NIH/3T3 cells, Raji cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15222-1-AP targets YWHAH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YWHAH fusion protein Ag7435
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC003047
• Gene Symbol: YWHAH
• Gene ID (NCBI): 7533
• RRID: AB_10863119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.; Authors - Jiarui Liu
human	WB,IF	Cell Biol Int; 14-3-3-η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage; Authors - Hui Zhou
rat	WB	Eur J Pharmacol; Capsaicin prevents mitochondrial damage, protects cardiomyocytes subjected to anoxia/reoxygenation injury mediated by 14-3-3η/Bcl-2.; Authors - Jiyi Huang
human,mouse	WB,IF,IP	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song ; KD Validated
rat	WB	Cells; Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development; Authors - Nghi M Nguyen
human	IF	J Hazard Mater; YTHDC2 mediated RNA m6A modification contributes to PM2.5-induced hepatic steatosis; Authors - Zhipeng Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vicki (Verified Customer) (01-13-2023)

Very expressive antibody used on BDEVs

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: EVs from Rat Brain

YWHAH Antibody Western Blot validation (1:2000 dilution) in EVs from Rat Brain (Cat no:15222-1-AP)

FH

Vicki (Verified Customer) (09-12-2022)

Works extremely well on hard to develop samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EVs from Rat Brain

YWHAH Antibody Western Blot validation (1:1000 dilution) in EVs from Rat Brain (Cat no:15222-1-AP)