YTHDF2 Recombinant antibody

• Featured Product
• KD/KO Validated

YTHDF2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3L6

Cat no : 81340-1-RR

Synonyms

NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14, CLL associated antigen KW 14

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Validation Data Gallery

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  WB analysis of Jurkat using 81340-1-RR

  WB result of YTHDF2 antibody (81340-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF2 transfected Jurkat cells.

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  WB analysis using 81340-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81340-1-RR (YTHDF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 81340-1-RR

  IP result of anti-YTHDF2 (IP:81340-1-RR, 4ug; Detection:81340-1-RR 1:1000) with HeLa cells lysate 1880 ug.

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  IF Staining of HeLa using 81340-1-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (81340-1-RR, Clone: 3L6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

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  IF Staining of HeLa using 81340-1-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (81340-1-RR, Clone: 3L6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81340-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81340-1-RR against Human YTHDF2 were performed. The affinity constant is 41.6 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells, Raji cells, NIH/3T3 cells, RAW 264.7 cells, C6 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81340-1-RR targets YTHDF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YTHDF2 fusion protein Ag5922
• Full Name: YTH domain family, member 2
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002559
• Gene Symbol: YTHDF2
• Gene ID (NCBI): 51441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.