YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 26771-1-AP

Synonyms

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

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Validation Data Gallery

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  WB analysis of Raji using 26771-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26771-1-AP (YTHDF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 26771-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26771-1-AP (YTHDF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26771-1-AP (YTHDF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 26771-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26771-1-AP (YTHDF2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 26771-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26771-1-AP (YTHDF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 26771-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26771-1-AP (YTHDF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 26771-1-AP is YTHDF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDF2 fusion protein Ag25348
• Full Name: YTH domain family, member 2
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002559
• Gene Symbol: YTHDF2
• Gene ID (NCBI): 51441
• RRID: AB_2880629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.

Publications

Species	Application	Title
mouse	WB	J Neurochem; METTL14 is decreased and regulates m6 A modification of α-synuclein in Parkinson's disease; Authors - Honghu He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yanhong (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HaCaT cells

YTHDF2 Antibody Western Blot, validation (1:1000 dilution) in HaCaT cells (Cat no:26771-1-AP)