YTHDF1 Recombinant antibody

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  WB analysis using 80876-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 80876-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293 using 80876-2-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.

YTHDF1 Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Cat No. 80876-2-RR

Clone No.241445B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 241445B10

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80876-2-RR targets YTHDF1 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YTHDF1 fusion protein Ag11509
• Full Name: YTH domain family, member 1
• Calculated Molecular Weight: 559 aa, 61 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC050284
• Gene Symbol: YTHDF1
• Gene ID (NCBI): 54915
• RRID: AB_3670491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BYJ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.